“A specific assay with high through-put and simple readout is the key to receive improved enzyme variants in a reasonable time-frame”
SeSaM-Biotech integrates biological, physical and chemical expertise in the development of highly specific assays to screen enzyme mutant libraries.
Depending on the desired through-put, the assays are designed on the following platforms:
| through-put | platform | readout |
| low | GC. HPLC | chromatogram (peaks) |
| medium | TLC, tube arrays | colorimetric |
| high | microtiter plate (96, 384 well) | colorimetric, fluorometric |
| very high | agar plate (semi-quantitative) | colorimetric, zone of clearance |
| ultra-high | FACS (semi-quantitative) | fluorometric |
Assay development includes test trials plus optimization of assay parameters and conditions.
Feel free to get in touch with us to learn about available assay systems and how we can support your R&D.