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Lessons from diversity of directed evolution experiments by an analysis of 3,000 mutations.
14 July 2014
Zhao J, Kardashliev T, Joelle
Biotechnol Bioeng 2014
Diversity generation by random mutagenesis is often the first key step in directed evolution...
read more >>
Bacillus gibsonii alkaline protease
1 November 2012
Martinez R, Jakob F, Tu R, Sie
Biotechnol Bioeng. 2013 Mar
Bacillus gibsonii Alkaline Protease (BgAP) is a recently reported subtilisin protease exhibiting...
read more >>
Review: To get what we aim for - progress in diversity generation methods
5 June 2013
Ruff AJ, Dennig A, Schwaneberg
FEBS J., 280 (2013), 2961-2978
Protein re-engineering by directed evolution has become a standard approach for tailoring enzymes...
read more >>
Reengineered glucose oxidase for amperometric glucose determination in diabetes analytics
20 June 2013
Arango Gutierrez E, Mundhada H
Biosensors and Bioelectronics
Glucose oxidase is an oxidoreductase exhibiting a high β-d-glucose specificity and high stability...
read more >>

News

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Lessons from diversity of directed evolution experiments by an analysis of 3,000 mutations.

Lessons from diversity of directed evolution experiments by an analysis of 3,000 mutations.
14 July 2014
Zhao J, Kardashliev T, Joelle Ruff A, Bocola M, Schwaneberg U
Biotechnol Bioeng 2014

Diversity generation by random mutagenesis is often the first key step in directed evolution experiments and screening of 1,000-2,000 clones is in most directed evolution campaigns sufficient to identify improved variants. For experimentalists important questions such as how many positions are mutated in the targeted gene and what amino acid substitutions can be expected after screening of 1,000-2,000 clones are surprisingly not answered by a statistical analysis of mutant libraries.

Thesis projects

Thesis projects
1 July 2014

SeSaM-Biotech offers industry related Thesis projects in the field of protein engineering to motivated students and applications are always welcome.
Please state your field of interest and your availability when applying, so we can try to adapt the project.

Please send your application as ONE pdf-file to: career[at]sesam-biotech.com

Internships

Internships
1 August 2014

SeSaM-Biotech is always looking for motivated students interested in an internship on a full-time basis (2-3 months). Please see the attachment for further information (in german).  

 

 

Bacillus gibsonii alkaline protease

Increasing activity and thermal resistance of Bacillus gibsonii alkaline protease (BgAP) by directed evolution.
1 November 2012
Martinez R, Jakob F, Tu R, Siegert P, Maurer KH, Schwaneberg U
Biotechnol Bioeng. 2013 Mar

Bacillus gibsonii Alkaline Protease (BgAP) is a recently reported subtilisin protease exhibiting activity and stability properties suitable for applications in laundry and dish washing detergents. However, BgAP suffers from a significant decrease of activity at low temperatures. In order to increase BgAP activity at 15°C, a directed evolution campaign based on the SeSaM random mutagenesis method was performed. An optimized microtiter plate expression system in B. subtilis was established and classical proteolytic detection methods were adapted for high throughput screening.

Review: To get what we aim for - progress in diversity generation methods

Review: To get what we aim for - progress in diversity generation methods
5 June 2013
Ruff AJ, Dennig A, Schwaneberg U
FEBS J., 280 (2013), 2961-2978

Protein re-engineering by directed evolution has become a standard approach for tailoring enzymes in many fields of science and industry. Advances in screening formats and screening systems are fueling progress and enabling novel directed evolution strategies, despite the fact that the quality of mutant libraries can still be improved significantly.

Reengineered glucose oxidase for amperometric glucose determination in diabetes analytics

Reengineered glucose oxidase for amperometric glucose determination in diabetes analytics
20 June 2013
Arango Gutierrez E, Mundhada H, Meier T, Duefel H, Bocola M, Schwaneberg U
Biosensors and Bioelectronics 50 (2013) 84–90

Glucose oxidase is an oxidoreductase exhibiting a high β-d-glucose specificity and high stability which renders glucose oxidase well-suited for applications in diabetes care. Nevertheless, GOx activity is highly oxygen dependent which can lead to inaccuracies in amperometric β-d-glucose determinations. Therefore a directed evolution campaign with two rounds of random mutagenesis (SeSaM followed by epPCR), site saturation mutagenesis studies on individual positions, and one simultaneous site saturation library (OmniChange; 4 positions) was performed.

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