In the end of a directed evolution project (see Directed evolution), the best enzyme variants are overexpressed using either SeSaM-Biotech proprietary strains or strains provided by our customers. Afterwards, the enzymes are purified following an internal, extensively validated protocol and eventually characterized for the properties they were optimized towards.
Properties we characterize enzymes for are:
- Michaelis-Menten parameters
- two-substrate competition
- substrate promiscuity
- stability in harsh conditions (high temperature, extreme pH values, high salt concentrations, organic solvent resistance etc.)
For characterization purposes we use a variety of assays that we develop for the given enzyme such as: