Contact Person

SeSaM-Biotech
Mr.
David
Schönauer
CEO
+49 241 938 569 79

News

Lessons from diversity of directed evolution experiments by an analysis of 3,000 mutations.
14 July 2014
Zhao J, Kardashliev T, Joelle
Biotechnol Bioeng 2014
Diversity generation by random mutagenesis is often the first key step in directed evolution...
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Bacillus gibsonii alkaline protease
1 November 2012
Martinez R, Jakob F, Tu R, Sie
Biotechnol Bioeng. 2013 Mar
Bacillus gibsonii Alkaline Protease (BgAP) is a recently reported subtilisin protease exhibiting...
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Review: To get what we aim for - progress in diversity generation methods
5 June 2013
Ruff AJ, Dennig A, Schwaneberg
FEBS J., 280 (2013), 2961-2978
Protein re-engineering by directed evolution has become a standard approach for tailoring enzymes...
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Reengineered glucose oxidase for amperometric glucose determination in diabetes analytics
20 June 2013
Arango Gutierrez E, Mundhada H
Biosensors and Bioelectronics
Glucose oxidase is an oxidoreductase exhibiting a high β-d-glucose specificity and high stability...
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Enzyme properties

Enzyme properties

In the end of a directed evolution project (see Directed evolution), the best enzyme variants are overexpressed using either SeSaM-Biotech proprietary strains or strains provided by our customers. Afterwards, the enzymes are purified following an internal, extensively validated protocol and eventually characterized for the properties they were optimized towards.
 
Properties we characterize enzymes for are:

  • Michaelis-Menten parameters
  • two-substrate competition
  • inhibition
  • substrate promiscuity
  • stability in harsh conditions (high temperature, extreme pH values, high salt concentrations, organic solvent resistance etc.)
  • etc.

For characterization purposes we use a variety of assays that we develop for the given enzyme such as:

  • colorimetric
  • fluorimetric
  • HPLC
  • GC
  • TLC
  • CD
  • etc.