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Lessons from diversity of directed evolution experiments by an analysis of 3,000 mutations.
14 July 2014
Zhao J, Kardashliev T, Joelle
Biotechnol Bioeng 2014
Diversity generation by random mutagenesis is often the first key step in directed evolution...
Bacillus gibsonii alkaline protease
1 November 2012
Martinez R, Jakob F, Tu R, Sie
Biotechnol Bioeng. 2013 Mar
Bacillus gibsonii Alkaline Protease (BgAP) is a recently reported subtilisin protease exhibiting...
Review: To get what we aim for - progress in diversity generation methods
5 June 2013
Ruff AJ, Dennig A, Schwaneberg
FEBS J., 280 (2013), 2961-2978
Protein re-engineering by directed evolution has become a standard approach for tailoring enzymes...
Reengineered glucose oxidase for amperometric glucose determination in diabetes analytics
20 June 2013
Arango Gutierrez E, Mundhada H
Biosensors and Bioelectronics
Glucose oxidase is an oxidoreductase exhibiting a high β-d-glucose specificity and high stability...

Evolution services

Evolution services

SeSaM-Biotech provides Directed Evolution of proteins within a R&D project. Each project will be designed individually to suit the needs of our customers and described in a detailed quote comprising 10-15 pages. Mutations are generated through SeSaM technology (random mutagenesis), OmniChange (focused mutagenesis) and guided by rational design based on computational simulations and analysis of the crystal structure or homology model of the enzyme.

Our enzyme evolution services include:
  • Consultation to find the best strategy to evolve the enzyme (random, rational, or combined approach)
  • Cloning & expression using our proprietary cloning technology and production strains
  • Sophisticated high throughput screening assay development for desired enzymatic reactions and their application
  • Design, generation and screening of the enzyme libraries
  • Optional: purification and characterization of best performing enzyme variants (kinetic properties, selectivity, stability etc.

Milestones and regular reports provide full control over the project's progress and maximum flexibility. Our standard business model ensures that our client retains full IP ownership of the evolved variants!

Please contact us to design a suitable enzyme evolution service for you.